Record Details

Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus.

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/5477/
 
Title Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus.
 
Creator Mohammad Kunhi, A. A.
Singh, R.
 
Subject 03 Fungi
05 Enzymes
 
Description Screening of several fungal cultures resulted in the selection of an isolate of Aspergillus candidus
which produced a considerable amount of RNA-degrading enzyme in both surface and submerged methods
of cultivation. The conditions for the assay of the RNAase were standardized at pH 4.5, 55 ~ and using
0.25 ~o yeast RNA as substrate. The enzyme was stable at pit 5.2. EDTA was found to activate the enzyme
slightly. At temperatures 50-- 60 ~ there was considerable loss in enzyme activity which was traced to the
presence of a contaminating protease which presumably degraded the RNAase optimally at this temperature.
The protease could be preferentially inactivated at or above 75 ~ The crude enzyme, in addition to RNAaso
was found to possess DNAase, nonspecific phosphodiesterase and 3'- and 5'-phosphomonoesterase activities.
 
Date 1981
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/5477/1/Folia%20Microbiol.%2026%2C%20328--333%20%281981%29.pdf
Mohammad Kunhi, A. A. and Singh, R. (1981) Standardisation of assay procedure and some properties of ribonuclease from Aspergillus candidus. Folia Microbiologica, 26 (4). pp. 328-333.