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Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L.

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Title Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L.
 
Creator Prakash, V.
Nandi, P. K.
Jirgensons, B.
 
Subject 03 Proteins
01 Oilseeds
 
Description The circular dichroic spectra of a-globulin have been obtained under various
solution conditions of sodium dodecyl sulfate, acid, alkali, urea and guanidine
hydrochloride. The protein in phosphate buffer pH 7.4, 0 . 2 , h~a s about 25% bstructure
and 5% a-helix, the rest being aperiodic or irregular structure. Sodium
dodecyl sulfate induced more a-helical structure in the protein. The protein had
nearly 20% a-helix at 1 x lo-= M SDS. At extreme acid or alkaline pH, the
protein had no a-helix with Pstructure decreasing with further extremes of pH.
The protein is represented by 100% aperiodic structure in 6.6 m urea and in 6.0 m guanidine hydrochloride solutions. The above results are discussed in view
of some of the earlier results with regard to the association-dissociation and
denaturation behavior of a-globulin under various solution conditions.
 
Date 1980
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/4006/1/Int.%20J.%20Peptide%20Protein%20Res.%2015%2C1980%2C305-313.pdf
Prakash, V. and Nandi, P. K. and Jirgensons, B. (1980) Effect of sodium dodecyl sulfate, acid, alkali, urea and guanidine hydrochloride on the circular dichroism of alpha-globulin of Sesamum indicum L. International Journal of Peptide and Protein Research, 15. pp. 305-313.