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Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress.

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Title Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress.
 
Creator Ravishankar, G. A.
Grewal, C.
 
Subject 10 Plants
05 Tissue Culture
 
Description Callus culture of Dioscorea deltoidea produced diosgenin and sterols during
stationary phase. Ammonium nitrate (420 mg Nitrogen/l) as sole nitrogen source
supported better growth than a combination of ammonium nitrate and potassium
nitrate (totally equivalent to 840 mg Nitrogen/l). The production of diosgenin
increased under low phosphate concentration (100 mg/l) whereas high phosphate
concentration (240 mg/!) promoted growth. Micronutrients, when used at 1ı89
strength, enhanced growth and diosgenin production. Depletion of nitrogen
increase in the diosgenin synthesis by a factor of 2. Adoption of a two stage
culture method enhanced the diosgenin production in cultured cells by eight-fold.
 
Date 1991
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/3769/1/Biotechnology%20Letters%20Vol%2013%20No%202%20125-130%20%281991%29.pdf
Ravishankar, G. A. and Grewal, C. (1991) Development of media for growth of Dioscorea deltoidea cells in in vitro diosgenin production: Influence of media constituents and nutrient stress. Biotechnology Letters, 13 (2). pp. 125-130.