Record Details

In vitro immunomodulatory potential of macromolecular components derived from the aqueous extract of ajowan [Trachyspermum ammi (L.) Sprague].

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/13499/
 
Title In vitro immunomodulatory potential of macromolecular components derived from the aqueous extract of ajowan [Trachyspermum ammi (L.) Sprague].
 
Creator Shruthi, R. R.
Venkatesh, Y. P.
Muralikrishna, G.
 
Subject 10 Immunology
30 Spices/Condiments
 
Description Ajowan [Trachyspermum ammi (L.) Sprague] has proven medicinal and nutritive properties. The role of ajowan within the intricate network of interaction of cells of the immune system has also been quite studied. In this present study, the immunomodulatory properties of polysaccharide/polysaccharide-protein complexes (PPCs) have been assessed.Ajowan aqueous extract was precipitated with ethanol followed by dialysis. The resultant dialyzed solution was subjected to DEAE-Cellulose column chromatography by stepwise elution with sodium chloride (0-0.5M); 0.4 M NaCl eluate showed effective mitogenic activity towards splenocytes and hence named as, ajowan immunomodulatory component (ImC). This component of interest comprised of acidic polysaccharides, glycoproteins and associated bound phenolics. ImC induced proliferation of murine splenocytes effectively at the concentration of 1 μg mL−1 (p < 0.005). Pronase treated ImC (Imc-Pt) failed to elicit mitogenic effect towards splenocytes; however, it could activate peritoneal exudate cells for synthesis of nitric oxide and phagocytosis at the minimum concentration of 10 ng mL−1 (p < 0.005). From the above results it can be concluded that both protein and polysaccharide constituents of ImC have independent roles in its immunomodulation property, i.e., protein on B-lymphocyte proliferation and carbohydrates on macrophage activation.
 
Date 2017
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/13499/1/IJTK%2016%283%29%20506-513.pdf
Shruthi, R. R. and Venkatesh, Y. P. and Muralikrishna, G. (2017) In vitro immunomodulatory potential of macromolecular components derived from the aqueous extract of ajowan [Trachyspermum ammi (L.) Sprague]. Indian Journal of Traditional Knowledge, 16 (3). pp. 506-513.