Record Details

Interaction of urea with the high molecular weight protein fraction, carmin, from safflower seed (Carthamus tinctorius).

IR@CSIR-CFTRI

View Archive Info
 
 
Field Value
 
Relation http://ir.cftri.com/2677/
 
Title Interaction of urea with the high molecular weight protein fraction, carmin, from safflower seed (Carthamus tinctorius).
 
Creator Prakash, V.
 
Subject 03 Proteins
01 Oilseeds
 
Description The effect of urea on the high molecular weight protein fraction, carmin, from
safflower seed Carthamus tinctorius L, was investigated by the techniques of
sedimentation velocity, viscosity, fluorescence and difference spectral measurements,
partial specific volume and circular dichroism. The protein underwent
dissociation and denaturation simultaneously in the presence of increasing concentration
of urea. The protein dissociated to a 2 s component and in the process,
the buried and exposed residues of tyrosines and tryptophans were perturbed.
At 8 M urea concentration, the protein existed completely as a random
coil; nearly 535 rnol of urea were bound per mol of protein. The results indicated
that the subunits of carmin appears to be held principally by noncovalent
interactions and are dissociated/denaturated by increasing concentration of urea,
leading to the destabilization of the native oligomer.
 
Date 1986
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2677/1/Int.%20J.%20Peptide%20Protein%20Res.%2028%2C%201986%2C%20192-200.pdf
Prakash, V. (1986) Interaction of urea with the high molecular weight protein fraction, carmin, from safflower seed (Carthamus tinctorius). International Journal of Peptide and Protein Research, 28. pp. 192-200.