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Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed.

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Title Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed.
 
Creator Mohan Reddy, I.
Narasinga Rao, M. S.
 
Subject 03 Proteins
03 Cotton seed
 
Description A method for the isolation of gossypin and congossypin from glanded cottonseed has been developed
on the basis of their solubility characteristics as a function of ionic strength and temperature. Further
purification of the proteins by gel filtration on Sephadex G-200 column yielded homogeneous (-98%)
preparations, as judged by sedimentation velocity, gel electrophoresis, and ion-exchange chromatography.
Gossypin and congossypin were characterized by sedimentation constants (S20,wof) 11.0 and 7.6 and
E;& at 280 nm of 7.6 and 6.0, respectively. The proteins were free from gossypol (free and bound) and
nucleic acid impurities.
 
Date 1988
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2711/1/Journal%20of%20Agricultural%20and%20Food%20Chemistry%2C%20Volume-36%282%20%281988%29%20237-240.pdf
Mohan Reddy, I. and Narasinga Rao, M. S. (1988) Method for the isolation of gossypin (11S protein) and congossypin (7S protein) from glanded cottonseed. Journal of Agricultural and Food Chemistry, 36 (2). pp. 237-240.