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A method for isolation of 2S, 7S, and 11S proteins of soyabean.

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Title A method for isolation of 2S, 7S, and 11S proteins of soyabean.
 
Creator Appu Rao, A. G.
Narasinga Rao, M. S.
 
Subject 03 Proteins
05 Soya bean
 
Description A method is described for the isolation of 2S, 7S and 11S proteins of soybean. The unfractionated proteins are first precipitated with Hg(II) which yields a 11S-rich precipitate and this is further purified by (NH4)2SO4precipitation. From Mg(II)supernatant which is rich in 7S and 2S proteins, they are separated by the use of (NH4)2SO4 and cold ethanol. The 7S and 11S protein are obtained in a highly purified form as indicated by ultracentrifugation and polyacrylamide gel electrophoresis.
 
Date 1977
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2476/1/PREPARATIVE%20BIOCHEMISTRY%2C%207%282%29%2C%2089-101%20%281977%29.pdf
Appu Rao, A. G. and Narasinga Rao, M. S. (1977) A method for isolation of 2S, 7S, and 11S proteins of soyabean. Preparative Biochemistry, 7 (2). pp. 89-101.