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A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.

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Relation http://ir.cftri.com/2275/
 
Title A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae.
 
Creator Shankar, C. S.
Umesh Kumar, S.
 
Subject 04 Yeast
 
Description A cell-wall-surface protein purified from the cells of Saccharomyces cerevisiae NCYC 227 was found to be involved in the non-sexual flocculation of this yeast. This 13 kDa protein was found to bind specifically to mannose. The protein bound to mannans isolated from yeast as well as in situ to intact cells, but only in the presence of calcium ions. The protein, a mannoprotein, formed aggregates as revealed in SDS-PAGE. Urea and higher temperatures prevented protein aggregation, suggesting that the flocculation of S. cerevisiae is primarily due to hydrogen bonding between mannan and protein.
 
Date 1994
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2275/1/Microbiology%20%281%20994%29%2C%20140%2C%201097-1%20101.pdf
Shankar, C. S. and Umesh Kumar, S. (1994) A surface lectin associated with flocculation in brewing strains of Saccharomyces cerevisiae. Microbiology, 140. pp. 1097-101. ISSN 1350-0872