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Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/2070/
 
Title Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization.
 
Creator Chowdary, G. V.
Prapulla, S. G.
 
Subject 04 Fungi
05 Enzymes
 
Description Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n-hexane. Response surface methodology based on five-level four-variable central composite rotatable design was employed to optimize four important reaction variables such as enzyme/substrate E/S ratio, substrate concentration, incubation time, and temperature affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; temperature, 60 degrees C. The predicted value matched well with experimentally obtained value of 487 mM.
 
Date 2003
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2070/1/Applied%20Biochemistry%20and%20Microbiology%2C%20Vol.%2039%2C%20No.%203%2C%202003%2C%20pp.%20243%E2%80%93248.pdf
Chowdary, G. V. and Prapulla, S. G. (2003) Synthesis of ethyl isovalerate using Rhizomucor miehei lipase: optimization. Applied Biochemistry and Microbiology, 39 (3). pp. 278-83.