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Reverse micellar extraction for downstream processing of proteins/enzymes.

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/2121/
 
Title Reverse micellar extraction for downstream processing of proteins/enzymes.
 
Creator Krishna, S. Hari
Srinivas, N. D.
Raghavarao, K. S. M. S.
Karanth, N. G.
 
Subject 05 Enzymes
05 Processing and Engineering
 
Description New developments in the area of downstream processing are, hopefully, to fulfill the promises of modern biotechnology. The traditional separation processes such as chromatography or electrophoresis can become prohibitively expensive unless the product is of high value. Hence, there is a need to develop efficient and cost-effective downstream processing methods. Reverse micellar extraction is one such potential and a promising liquid-liquid extraction technique, which has received immense attention for isolation and purification of proteins/enzymes in the recent times. This technique is easy to scale-up and offers continuous operation. This review, besides briefly considering important physico-chemical and biological aspects, highlights the engineering aspects including mass transfer, mathematical modeling, and technology development. It also discusses recent developments in reverse micellar extraction such as affinity based separations, enzymatic reactions in reverse micelles coupled with membrane processes, reverse micellar extraction in hollow fibers, etc. Special emphasis has been given to some recent applications of this technique.
 
Date 2002
 
Type Article
PeerReviewed
 
Format pdf
 
Language en
 
Identifier http://ir.cftri.com/2121/1/Advances%20in%20biochemical%20engineeringbiotechnology%2C%20Volume-75%28%20%282002%29%20119-83.pdf
Krishna, S. Hari and Srinivas, N. D. and Raghavarao, K. S. M. S. and Karanth, N. G. (2002) Reverse micellar extraction for downstream processing of proteins/enzymes. Advances in Biochemical Engineering/Biotechnology, 75. pp. 119-83. ISSN 0724-6145