Record Details


Field	Value

Relation	http://ir.cftri.com/2232/

Title	MIG1 overexpression causes flocculation in Saccharomyces cerevisiae.

Creator	Shankar, C. S. Ramakrishnan, M. S. Umesh Kumar, S.

Subject	19 Yeast 04 Fermentation Technology

Description	MIG1, encoding a C2H2 zinc-finger repressor protein involved in carbon catabolite repression, was found to play a role in non-sexual flocculation of Saccharomyces cerevisiae. Disruption of MIG1 in a flocculent mutant strain of NCYC 227, resulted in a non-flocculent phenotype. Expression of MIG1 on a 2 mu pRS426 vector in a non-flocculent strain, YM 4134, caused flocculation; MIG1 on a high-copy-number LEU2-d plasmid caused intense flocculation in the same strain. Mutations in the SSN6 and TUP1 genes confer a flocculent phenotype in non-flocculent strains of S. cerevisiae, and it has been shown that Mig1 can tether the Ssn6p-Tup1p complex to the regulatory regions of glucose-repressible genes. Mutations in tup1 in a MIG1 background caused flocculation while double mutants of TUP1 and MIG1 did not flocculate. Based on these results, a model for the role of MIG1 in flocculation gene regulation is proposed.

Date	1996

Type	Article PeerReviewed

Format	pdf

Language	en

Identifier	http://ir.cftri.com/2232/1/Microbiology%201996%201%2C%20142%2C2663-2667.pdf Shankar, C. S. and Ramakrishnan, M. S. and Umesh Kumar, S. (1996) MIG1 overexpression causes flocculation in Saccharomyces cerevisiae. Microbiology, 142 (. pp. 2663-7. ISSN 1350-0872

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