Record Details

Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii
 
Creator Khatri, Indu
Tomar, Rajul
Ganesan, K.
Prasad, G. S.
Subramanian, Srikrishna
 
Subject QR Microbiology
 
Description The probiotic yeast, Saccharomyces boulardii (Sb) is known to be effective against many gastrointestinal disorders and antibiotic-associated diarrhea. To understand molecular basis of probiotic-properties ascribed to Sb we determined the complete genomes of two strains of Sb i.e. Biocodex and unique28 and the draft genomes for three other Sb strains that are marketed as probiotics in India. We compared these genomes with 145 strains of S. cerevisiae (Sc) to understand genome-level similarities and differences between these yeasts. A distinctive feature of Sb from other Sc is absence of Ty elements Ty1, Ty3, Ty4 and associated LTR. However, we could identify complete Ty2 and Ty5 elements in Sb. The genes for hexose transporters HXT11 and HXT9, and asparagine-utilization are absent in all Sb strains. We find differences in repeat periods and copy numbers of repeats in flocculin genes that are likely related to the differential adhesion of Sb as compared to Sc. Core-proteome based taxonomy places Sb strains along with wine strains of Sc. We find the introgression of five genes from Z. bailii into the chromosome IV of Sb and wine strains of Sc. Intriguingly, genes involved in conferring known probiotic properties to Sb are conserved in most Sc strains.
 
Publisher Nature Publishing Group
 
Date 2017
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1038/s41598-017-00414-2
http://crdd.osdd.net/open/1969/
 
Identifier Khatri, Indu and Tomar, Rajul and Ganesan, K. and Prasad, G. S. and Subramanian, Srikrishna (2017) Complete genome sequence and comparative genomics of the probiotic yeast Saccharomyces boulardii. Scientific Reports, 7. ISSN 2045-2322