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Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture
 
Creator Priyanka, Anu
Solanki, Vipul
Parkesh, Raman
Thakur, Krishan Gopal
 
Subject QR Microbiology
 
Description Human SIRT7 is an NAD(+) dependent deacetylase, which belongs to sirtuin family of proteins. SIRT7, like other sirtuins has conserved catalytic domain and is flanked by N- and C-terminal domains reported to play vital functional roles. Here, we report the crystal structure of the N-terminal domain of human SIRT7 (SIRT7(NTD) ) at 2.3 Å resolution as MBP-SIRT7(NTD) fusion protein. SIRT7(NTD) adopts three-helical domain architecture and comparative structural analyses suggest similarities to some DNA binding motifs and transcription regulators. We also report here the importance of N- and C-terminal domains in soluble expression of SIRT7. Proteins 2016; 84:1558-1563.
 
Publisher Wiley
 
Date 2016
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1002/prot.25085
http://crdd.osdd.net/open/1986/
 
Identifier Priyanka, Anu and Solanki, Vipul and Parkesh, Raman and Thakur, Krishan Gopal (2016) Crystal structure of the N-terminal domain of human SIRT7 reveals a three-helical domain architecture. Proteins: Structure, Function, and Bioinformatics, 84 (10). pp. 1558-1563. ISSN 08873585