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In silico analysis to identify vaccine candidates common to multiple serotypes of Shigella and evaluation of their immunogenicity

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title In silico analysis to identify vaccine candidates common to multiple serotypes of Shigella and evaluation of their immunogenicity
 
Creator Ahmed, Niyaz
Pahil, Sapna
Taneja, Neelam
Ansari, Hifzur Rahman
Raghava, G.P.S.
 
Subject QR Microbiology
 
Description Shigellosis or bacillary dysentery is an important cause of diarrhea, with the majority of the cases occurring in developing countries. Considering the high disease burden, increasing antibiotic resistance, serotype-specific immunity and the post-infectious sequelae associated with shigellosis, there is a pressing need of an effective vaccine against multiple serotypes of the pathogen. In the present study, we used bio-informatics approach to identify antigens shared among multiple serotypes of Shigella spp. This approach led to the identification of many immunogenic peptides. The five most promising peptides based on MHC binding efficiency were a putative lipoprotein (EL PGI I), a putative heat shock protein (EL PGI II), Spa32 (EL PGI III), IcsB (EL PGI IV) and a hypothetical protein (EL PGI V). These peptides were synthesized and the immunogenicity was evaluated in BALB/c mice by ELISA and cytokine assays. The putative heat shock protein (HSP) and the hypothetical protein elicited good humoral response, whereas putative lipoprotein, Spa32 and IcsB elicited good T-cell response as revealed by increased IFN-γ and TNF-α cytokine levels. The patient sera from confirmed cases of shigellosis were also evaluated for the presence of peptide specific antibodies with significant IgG and IgA antibodies against the HSP and the hypothetical protein, bestowing them as potential future vaccine candidates. The antigens reported in this study are novel and have not been tested as vaccine candidates against Shigella. This study offers time and cost-effective way of identifying unprecedented immunogenic antigens to be used as potential vaccine candidates. Moreover, this approach should easily be extendable to find new potential vaccine candidates for other pathogenic bacteria.
 
Publisher Public Library of Science
 
Date 2017
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1371/journal.pone.0180505
http://crdd.osdd.net/open/2027/
 
Identifier Ahmed, Niyaz and Pahil, Sapna and Taneja, Neelam and Ansari, Hifzur Rahman and Raghava, G.P.S. (2017) In silico analysis to identify vaccine candidates common to multiple serotypes of Shigella and evaluation of their immunogenicity. PLOS ONE, 12 (8). e0180505. ISSN 1932-6203