Record Details

Microbial F-type lectin domains with affinity for blood group antigens

DIR@IMTECH: CSIR-Institute of Microbial Technology

View Archive Info
 
 
Field Value
 
Title Microbial F-type lectin domains with affinity for blood group antigens
 
Creator Mahajan, Sonal
Khairnar, Aasawari
Bishnoi, Ritika
Ramya, T.N.C.
 
Subject QR Microbiology
 
Description F-type lectins are fucose binding lectins with characteristic fucose binding and calcium binding motifs. Although they occur with a selective distribution in viruses, prokaryotes and eukaryotes, most biochemical studies have focused on vertebrate F-type lectins. Recently, using sensitive bioinformatics search techniques on the non-redundant database, we had identified many microbial F-type lectin domains with diverse domain organizations. We report here the biochemical characterization of F-type lectin domains from Cyanobium sp. PCC 7001, Myxococcus hansupus and Leucothrix mucor. We demonstrate that while all these three microbial F-type lectin domains bind to the blood group H antigen epitope on fucosylated glycans, there are fine differences in their glycan binding specificity. Cyanobium sp. PCC 7001 F-type lectin domain binds exclusively to extended H type-2 motif, Myxococcus hansupus F-type lectin domain binds to B, H type-1 and Lewisb motifs, and Leucothrix mucor F-type lectin domain binds to a wide range of fucosylated glycans, including A, B, H and Lewis antigens. We believe that these microbial lectins will be useful additions to the glycobiologist's toolbox for labeling, isolating and visualizing glycans.
 
Publisher Elsevier
 
Date 2017
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1016/j.bbrc.2017.07.125
http://crdd.osdd.net/open/2063/
 
Identifier Mahajan, Sonal and Khairnar, Aasawari and Bishnoi, Ritika and Ramya, T.N.C. (2017) Microbial F-type lectin domains with affinity for blood group antigens. Biochemical and Biophysical Research Communications, 491 (3). pp. 708-713. ISSN 0006291X