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Cobalt and nickel impair DNA metabolism by the oxidative stress independent pathway

DIR@IMTECH: CSIR-Institute of Microbial Technology

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Title Cobalt and nickel impair DNA metabolism by the oxidative stress independent pathway
 
Creator Kumar, Vineet
Mishra, Rajesh Kumar
Kaur, Gursharan
Dutta, Dipak
 
Subject QR Microbiology
 
Description The oxidative stress that evolves under cobalt and nickel exposure is thought to exert toxicity, though the exact routes of such metal poisoning remain ambiguous. We revisited the metal toxicity in Escherichia coli to show that cobalt and nickel exposure at levels as low as 0.5 and 1 mM, respectively, visibly inhibits growth. We also observed that acidic conditions aggravated, while alkaline conditions alleviated the metal toxicity. Besides, 1 mM manganese, which is non-cytotoxic, as judged by the growth of E. coli, synergistically elevated cobalt and nickel stress. However, the metal toxicity did not lead to oxidative stress in E. coli. On the other hand, we show that cobalt and nickel, but not manganese, reduced the rate of DNA replication to 50% within 2 hours. Interestingly, the metal ions promoted DNA double-strand breaks but did not induce SOS repair pathways, indicating that the metal ions could block SOS induction. To test this, we show that cobalt and nickel, but not manganese, suppressed the nalidixic acid-induced SOS response. Finally, using an in vitro assay system, we demonstrated that cobalt and nickel inhibit RecBCD function, which is essential for SOS induction. Therefore, our data indicate that cobalt and nickel affect DNA replication, damage DNA, and inhibit the SOS repair pathway to exert toxicity.
 
Publisher RSC
 
Date 2017
 
Type Article
PeerReviewed
 
Relation http://dx.doi.org/10.1039/c7mt00231a
http://crdd.osdd.net/open/2069/
 
Identifier Kumar, Vineet and Mishra, Rajesh Kumar and Kaur, Gursharan and Dutta, Dipak (2017) Cobalt and nickel impair DNA metabolism by the oxidative stress independent pathway. Metallomics, 9 (11). pp. 1596-1609. ISSN 1756-5901