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Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae)

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Relation http://oar.icrisat.org/10532/
http://dx.doi.org/10.1007/s10863-017-9739-3
10.1007/s10863-017-9739-3
 
Title Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae)
 
Creator Nareshkumar, B
Akbar, S M
Sharma, H C
Jayalakshmi, S K
Sreeramulu, K
 
Subject Pest Management
Helicoverpa
Entomology
 
Description Neonicotinoids have high agonistic affinity to insect nicotinic acetylcholine receptors (nAChR) and are frequently used as insecticides against most devastating lepidopteran insect pests. Imidacloprid influenced dose-dependent decline in the state III and IV respiration, respiration control index (RCI), and P/O ratios, in vitro and in vivo. The bioassay indicated its LD50 value to be 531.24 μM. The insecticide exhibited a dose-dependent inhibition on F0F1-ATPase and complex IV activity. At 600 μM, the insecticide inhibited 83.62 and 27.13% of F0F1-ATPase and complex IV activity, respectively, and induced the release of 0.26 nmoles/min/mg protein of cytochrome c. A significant dose- and time-dependent increase in oxidative stress was observed; at 600 μM, the insecticide correspondingly induced lipid peroxidation, LDH activity, and accumulation of H2O2 content by 83.33, 31.51 and 223.66%. The stress was the maximum at 48 h of insecticide treatment (91.58, 35.28, and 189.80%, respectively). In contrast, catalase and superoxide dismutase were reduced in a dose- and time-dependent manner in imidacloprid-fed larvae. The results therefore suggest that imidacloprid impedes mitochondrial function and induces oxidative stress in H. armigera, which contributes to reduced growth of the larvae along with its neurotoxic effect.
 
Publisher Springer
 
Date 2018-02
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10532/1/Imidacloprid%20impedes%20mitochondrial%20function%20and%20induces%20oxidative.pdf
Nareshkumar, B and Akbar, S M and Sharma, H C and Jayalakshmi, S K and Sreeramulu, K (2018) Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae). Journal of Bioenergetics and Biomembranes (TSI), 50 (1). pp. 21-32. ISSN 0145-479X