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Quantification of Amylase Enzyme from the Calcite Residing Actinobacteria Isolated from Limestone Quarries of Deccan Traps

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Relation http://oar.icrisat.org/10584/
 
Title Quantification of Amylase Enzyme from the Calcite Residing Actinobacteria Isolated from Limestone Quarries of Deccan Traps
 
Creator Quadri, S R
Ruwaili, J A
Akbar, S M
Li, W J
Agsar, D
 
Subject Entomology
 
Description Sixty three actinobacteria were isolated from Limestone quarries of Deccan
Traps from South India. The isolates were identified by morphological, biochemical and
physiological studies. Among these, six isolates were selected on the basis of their
amylolytic activity using submerged cultivation. The potential isolates exhibiting
amylolytic activity were DRQ10, DRQ20, DRQ86, DRQ33, DRQ41, and DRQ63 as confirmed
by formation of clear zones of starch hydrolysis around the colonies, with maximum
activities 2500, 1100, 1200, 1150, 1250, 1400, and 1600 Units, respectively, as determined
by DNS (3, 5-dinitrosalicylic acid) assay method. Various physicochemical parameters
such as pH, temperature, incubation time were optimized for the production of amylase.
Amylase production was maximum on 4th day of incubation. The optimum pH and
temperature were found to be 9.0 and 45 oC, and NaCl concentration of 3M for the potential
amylolytic isolates. The carbon and nitrogen source for the production of amylase was
starch and peptone, respectively.
 
Publisher Oriental Scientific Pub Co
 
Date 2014-11
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10584/1/Syed%20Raziuddin%20Quadri.pdf
Quadri, S R and Ruwaili, J A and Akbar, S M and Li, W J and Agsar, D (2014) Quantification of Amylase Enzyme from the Calcite Residing Actinobacteria Isolated from Limestone Quarries of Deccan Traps. Journal of Pure and Applied Microbiology, 8 (2). pp. 357-362. ISSN 0973-7510