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The RNA-Seq based high resolution gene expression atlas of chickpea (Cicer arietinum L.) reveals dynamic spatio-temporal changes associated with growth and development

OAR@ICRISAT

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Relation http://oar.icrisat.org/10623/
http://dx.doi.org/10.1111/pce.13210
10.1111/pce.13210
 
Title The RNA-Seq based high resolution gene expression atlas of chickpea (Cicer arietinum L.) reveals dynamic spatio-temporal changes associated with growth and development
 
Creator Kudapa, H
Garg, V
Chitikineni, A
Varshney, R K
 
Subject Abiotic Stress
Crop Improvement
Chickpea
Drought
Genetics and Genomics
Plant Growth
 
Description Chickpea is one of the world's largest cultivated food legume and is an excellent source of high‐quality protein to the human diet. Plant growth and development are controlled by programmed expression of a suite of genes at the given time, stage and tissue. Understanding how the underlying genome sequence translates into specific plant phenotypes at key developmental stages, information on gene expression patterns is crucial. Here we present a comprehensive Cicer arietinum Gene Expression Atlas (CaGEA) across the plant developmental stages and organs covering the entire life cycle of chickpea. One of the widely used drought tolerant cultivar, ICC 4958 has been used to generate RNA‐Seq data from 27 samples at five major developmental stages of the plant. A total of 816 million raw reads were generated and of these, 794 million filtered reads after QC were subjected to downstream analysis. A total of 15,947 unique number of differentially expressed genes across different pairwise tissue combinations were identified. Significant differences in gene expression patterns contributing in the process of flowering, nodulation, seed and root development were inferred in this study. Furthermore, differentially expressed candidate genes from “QTL‐hotspot” region associated with drought stress response in chickpea were validated.
 
Publisher John Wiley & Sons
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10623/1/Kudapa_et_al-2018-Plant%252C_Cell_%2526_Environment.pdf
Kudapa, H and Garg, V and Chitikineni, A and Varshney, R K (2018) The RNA-Seq based high resolution gene expression atlas of chickpea (Cicer arietinum L.) reveals dynamic spatio-temporal changes associated with growth and development. Plant, Cell & Environment (TSI). pp. 1-17. ISSN 0140-7791