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Groundnut (Arachis hypogaea L.) and cowpea (Vigna unguiculata L. Walp) growing in Ethiopia are nodulated by diverse rhizobia

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Relation http://oar.icrisat.org/10641/
http://dx.doi.org/10.5897/AJMR2017.8756
10.5897/AJMR2017.8756
 
Title Groundnut (Arachis hypogaea L.) and cowpea (Vigna unguiculata L. Walp) growing in Ethiopia are nodulated by diverse rhizobia
 
Creator Degefu, T
Wolde-meskel, E
Ataro, Z
Fikre, A
Amede, T
Ojiewo, C O
 
Subject Cowpea
Groundnut
Genetics and Genomics
Ethiopia
 
Description A total of eighty one (81) rhizobial isolates were recovered from root nodules of cowpea (Vigna
unguiculata L. Walp.) and groundnut (Arachis hypogaea L.) grown in soils collected from eight different
sites (Hawassa, Wondogenet, Chofa, Badawacho, Bodity, Gofa, Ziway, and Alemtena) in Ethiopia with
no known history of inoculation. The test isolates together with seven reference strains belonging to
five genera including Rhizobium, Ensifer, Mesorhizobium, Bradyrhizobium and Azorhizobium were
characterized using ninety phenotypic traits. Thirty one isolates (38%) were found to be fast growers
while fifty isolates (62%) were slow growers. The majority of the isolates showed an intrinsic resistance
to antibiotics (μg/ml), Chloramphenicol (5 and 15), Lincomycin (100), Novobiocin (0.5 and 1.5), and
Erythromycin (10 and 20) and to heavy metals manganese sulphate (500) and copper chloride (100).
Most isolates did not tolerate NaCl concentration >3% (w/v) and high temperature (45°C). Dendrogram
was constructed by applying the unweighted pair group method with arithmetic means (UPGMA) using
NTSYSpc Version 2.1. They were grouped into seven clusters and eight unclustered positions, when
82% relative similarity was used as a cut point. Fifty eight percent of the test isolates were grouped with
Bradyrhizobium japonicum and Bradyrhizobium elkanii superclades, thus indicating that rhizobia
nodulating cowpea and groundnut are delineated within a branch that defines Bradyrhizobium genus.
To elucidate the precise taxonomic positions of the isolates, further genetic studies are required using
modern molecular biological methods.
 
Publisher Academic Journals
 
Date 2018-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10641/1/Tulu%20Groundnut%20and%20Cowpea.pdf
Degefu, T and Wolde-meskel, E and Ataro, Z and Fikre, A and Amede, T and Ojiewo, C O (2018) Groundnut (Arachis hypogaea L.) and cowpea (Vigna unguiculata L. Walp) growing in Ethiopia are nodulated by diverse rhizobia. African Journal of Microbiology Research, 12 (9). pp. 200-217. ISSN 1996-0808