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Molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea (Cajanus cajan (L.) Millsp.)

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Relation http://oar.icrisat.org/10661/
http://dx.doi.org/10.1007/s00122-018-3101-y
10.1007/s00122-018-3101-y
 
Title Molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea (Cajanus cajan (L.) Millsp.)
 
Creator Saxena, R K
Patel, K
Sameer Kumar, C V
Tyagi, K
Saxena, K B
Varshney, R K
 
Subject Molecular Biology
Plant Breeding
Pigeonpea
 
Description Key message We report molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in
pigeonpea. We have also developed PCR-based markers amenable to low-cost genotyping to identify fertility restorer
lines.
Abstract Commercial hybrids in pigeonpea are based on A4 cytoplasmic male sterility (CMS) system, and their fertility
restoration is one of the key prerequisites for breeding. In this context, an effort has been made to understand the genetics and
identify quantitative trait loci (QTL) associated with restoration of fertility (Rf). One F2
population was developed by crossing
CMS line (ICPA 2039) with fertility restorer line (ICPL 87119). Genetic analysis has shown involvement of two dominant
genes in regulation of restoration of fertility. In parallel, the genotyping-by-sequencing (GBS) approach has generated ~ 33 Gb
data on the F2
population. GBS data have provided 2457 single nucleotide polymorphism (SNPs) segregating across the mapping
population. Based on these genotyping data, a genetic map has been developed with 306 SNPs covering a total length
981.9 cM. Further QTL analysis has provided the region flanked by S8_7664779 and S8_6474381 on CcLG08 harboured
major QTL explained up to 28.5% phenotypic variation. Subsequently, sequence information within the major QTLs was
compared between the maintainer and the restorer lines. From this sequence information, we have developed two PCR-based
markers for identification of restorer lines from non-restorer lines and validated them on parental lines of hybrids as well
as on another F2
mapping population. The results obtained in this study are expected to enhance the efficiency of selection
for the identification of restorer lines in hybrid breeding and may reduce traditional time-consuming phenotyping activities.
 
Publisher Springer
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10661/1/Saxena2018_Article_MolecularMappingAndInheritance.pdf
Saxena, R K and Patel, K and Sameer Kumar, C V and Tyagi, K and Saxena, K B and Varshney, R K (2018) Molecular mapping and inheritance of restoration of fertility (Rf) in A4 hybrid system in pigeonpea (Cajanus cajan (L.) Millsp.). Theoretical and Applied Genetics (TSI). pp. 1-10. ISSN 0040-5752