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A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence

OAR@ICRISAT

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Relation http://oar.icrisat.org/10667/
http://dx.doi.org/10.1007/s11103-018-0728-7
10.1007/s11103-018-0728-7
 
Title A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence
 
Creator Bhatnagar-Mathur, P
Gupta, R
Reddy, P S
Reddy, B P
Reddy, D S
Sameer Kumar, C V
Saxena, R K
Sharma, K K
 
Subject Pigeonpea
Genetics and Genomics
 
Description Key message
A novel open reading frame (ORF) identified and cloned from the A4 cytoplasm of Cajanus cajanifolius induced partial to complete male sterility when introduced into Arabidopsis and tobacco.

Abstract
Pigeonpea (Cajanus cajan L. Millsp.) is the only legume known to have commercial hybrid seed technology based on cytoplasmic male sterility (CMS). We identified a novel ORF (orf147) from the A4 cytoplasm of C. cajanifolius that was created via rearrangements in the CMS line and co-transcribes with the known and unknown sequences. The bi/poly-cistronic transcripts cause gain-of-function variants in the mitochondrial genome of CMS pigeonpea lines having distinct processing mechanisms and transcription start sites. In presence of orf147, significant repression of Escherichia coli growth indicated its toxicity to the host cells and induced partial to complete male sterility in transgenic progenies of Arabidopsis thaliana and Nicotiana tabacum where phenotype co-segregated with the transgene. The male sterile plants showed aberrant floral development and reduced lignin content in the anthers. Gene expression studies in male sterile pigeonpea, Arabidopsis and tobacco plants confirmed down-regulation of several anther biogenesis genes and key genes involved in monolignol biosynthesis, indicative of regulation of retrograde signaling. Besides providing evidence for the involvement of orf147 in pigeonpea CMS, this study provides valuable insights into its function. Cytotoxicity and aberrant programmed cell death induced by orf147 could be important for mechanism underlying male sterility that offers opportunities for possible translation for these findings for exploiting hybrid vigor in other recalcitrant crops as well.
 
Publisher Springer
 
Date 2018-05
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10667/1/A%20novel%20mitochondrial%20orf147%20causes%20cytoplasmic%20male%20sterility.pdf
Bhatnagar-Mathur, P and Gupta, R and Reddy, P S and Reddy, B P and Reddy, D S and Sameer Kumar, C V and Saxena, R K and Sharma, K K (2018) A novel mitochondrial orf147 causes cytoplasmic male sterility in pigeonpea by modulating aberrant anther dehiscence. Plant Molecular Biology (TSI), 97 (1-2). pp. 131-147. ISSN 0167-4412