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Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis

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Relation http://oar.icrisat.org/10685/
http://dx.doi.org/10.5897/AJB2018.16452
doi:10.5897/AJB2018.16452
 
Title Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis
 
Creator Alex, N
Cecilia, M
Mathew, N
Asunta, M
Henry, O
Wilton, M
 
Subject Pearl Millet
Plant Physiology
Plant Growth
East Africa
 
Description In the present study, an efficient protocol for somatic embryogenesis and plant regeneration was established in six finger millet varieties (GBK-043137, GBK-043128, GBK-043124, GBK-043122, GBK- 043094 and GBK-043050). Shoot tips from 3 days in vitro grown plants were inoculated on MS supplemented with various concentrations and combinations of α-naphthaleneacetic acid (NAA), 2,4- Dichlorophenoxyacetic acid (2,4-D), benzylaminopurine (BAP) and kinetin for callus induction and somatic embryogenesis. For shoot regeneration, somatic embryos were cultured on various concentrations of BAP, while root induction was done using different concentrations and combinations of NAA, kinetin, BAP and 2,4-D. Acclimatization of regenerated plants was tested using forest soil, cocopeat, manure, sand and fertilizer either singly or in combination. Best callus formation was achieved on 2.5 mg/l of 2,4-D and 1.5 mg/l BAP with a mean of 12.33±0.33 on variety GBK-043128 while shooting and rooting were best on 1.75 mg/l BAP with a mean of 25.07±0.64 and 1.0 BAP+0.25 NAA with a mean of 15.00±2.2, respectively. Best acclimatization was attained using soil, sand and fertilizer on GBK-043094. Plants regenerated were morphologically similar to in vivo plants with 97% survival rate. Moreover, they were fertile and able to set viable seeds. This efficient protocol has the potential for crop improvement and genomic studies.
 
Publisher Academic Journals
 
Date 2018-05-23
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10685/1/A026A6657193.pdf
Alex, N and Cecilia, M and Mathew, N and Asunta, M and Henry, O and Wilton, M (2018) Efficient plant regeneration protocol for finger millet [Eleusine coracana (L.) Gaertn.] via somatic embryogenesis. African Journal of Biotechnology, 17 (21). pp. 660-667. ISSN 1684-5315