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Phenotypic and genetic dissection of water stress adaptations in pearl millet (Pennisetum glaucum)

OAR@ICRISAT

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Relation http://oar.icrisat.org/10726/
 
Title Phenotypic and genetic dissection of water stress adaptations in pearl millet (Pennisetum glaucum)
 
Creator Tharanya, M
Kholova, J
Sivasakthi, K
Vadez, V
Seghal, D
Hash, C T
Raj, B
Baddam, R
Thirunalasundari, T
Yadav, R
 
Subject Abiotic Stress
Pearl Millet
Genetics and Genomics
 
Description Pearl millet is an important staple food for farming communities
across semi-arid tropical systems of South Asia and
Sub-Saharan Africa where production suffers uncertain precipitation.
This work is undertaken under the premise that maximizing
grain yield under water-limited conditions depends
on both maximizing water use and ensuring water availability
for the grain filling period. Here we discuss the phenotyping
methods targeting the variability in plant water use strategies
which determine the crop production success in water-limited
environments. A fine-mapping population of pearl millet,
segregating within the previously identified drought tolerance
quantitative trait locus (QTL) on chromosome 2 (LG02), was
tested across different experimental environments (pot culture,
high-throughput phenotyping platform (LeasyScan), Lysimeter,
and Field). Recombinants were then analyzed for traits
at different levels of plant organization, ranging from water-use
traits (transpiration rate, leaf area, plant organ dry weights,
etc.) to crop production and agronomic traits (grain yield, tiller
number, harvest index, etc.) The linkages between traits
across the experimental systems were analyzed, using principal
component analysis (PCA) and QTL co-localization approach.
The functional relevance of the phenotyping systems was traced
by PCA analysis. Furthermore, we found four regions within the
LG02-QTL underlying substantial co-mapping of water-use related
and agronomic traits. These regions were identified across
the experimental systems and justified linkages between water-use
traits were phenotyped at lower level of plant organization
to the agronomic traits assessed in the field. Therefore, the
phenotyping systems at ICRISAT are validated and well set to
accelerate crop breeding for drought adaptations.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10726/1/298.pdf
Tharanya, M and Kholova, J and Sivasakthi, K and Vadez, V and Seghal, D and Hash, C T and Raj, B and Baddam, R and Thirunalasundari, T and Yadav, R (2017) Phenotypic and genetic dissection of water stress adaptations in pearl millet (Pennisetum glaucum). In: InterDrought-V, February 21-25, 2017, Hyderabad, India.