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Molecular mapping of seed protein content in pigeonpea – a drought tolerant crop of the semi-arid tropics

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Relation http://oar.icrisat.org/10727/
 
Title Molecular mapping of seed protein content in pigeonpea – a drought tolerant crop of the semi-arid tropics
 
Creator Obala, J
Saxena, R K
Singh, V K
Kale, S M
Garg, V
Kumar, V
Sameer Kumar, C V
Tongoona, P
Sibiya, J
Saxena, K B
Varshney, R K
 
Subject Molecular Biology
Semi-arid tropics
Pigeonpea
 
Description Pigeonpea is a uniquely drought and heat tolerant crop that provides
a major source of dietary protein to nearly a billion people
in the tropical and semi-arid tropics of the world. Despite its importance
as a source of dietary protein the genetic control of its
seed protein content (SPC) is poorly understood. The present
study used high density linkage maps to identify quantitative
trait loci (QTL) associated with SPC and its relationship with
100-seed weight (HSW), seed yield (SY), days to first flower
(DTFF) and growth habit (GH). Five F2 mapping populations
segregating for SPS including ICP 11605 × ICP 14209, ICP 8863
× ICP 11605, HPL 24 × ICP 11605, ICP 5529 × ICP 11605 and
ICP 8863 × ICPL 87119 were genotyped using genotyping-by-sequencing
and phenotyped for the traits. The average inter-marker
distance on the population-specific maps varied from 1.6cM
to 3.5cM.On the basis of the population-specific and consensus
linkage maps, 48main effect QTLs (M-QTLs)with phenotypic
variance explained (PVE) ranging from 0.7 to 23.5% were detected
across five populations, of which 15 M-QTLs were major
(PVE≥10). Twenty seven of the M-QTLs could be collapsed
into six consensus QTL regions. In addition, 34 epistatic QTLs
(E-QTLs) with PVE ranging from 6.3% to 69.8% were detected
across populations. Co-localization of M-QTLs and E-QTLs affecting
SPC and the agronomic traits explained the genetic basis
of the significant (P < 0.05) correlations of SPC with HSW (r2 =
0.22 to 0.30), SY (r2 = -0.18 to -0.28), DTFF (r2 = -0.17 to -0.31)
and GH (r2 = 0.18 to 0.34). The quantitative nature of genetic
control of SPC and its relationship with agronomic traits suggest
that marker-assisted recurrent selection or genomic selection
would be effective for the simultaneous improvement of SPC
and other important traits.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/10727/1/302.pdf
Obala, J and Saxena, R K and Singh, V K and Kale, S M and Garg, V and Kumar, V and Sameer Kumar, C V and Tongoona, P and Sibiya, J and Saxena, K B and Varshney, R K (2017) Molecular mapping of seed protein content in pigeonpea – a drought tolerant crop of the semi-arid tropics. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.