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Isolation and characterization of stress inducible promoters from Pennisetum glaucum and their role in abiotic stress adaptation

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Relation http://oar.icrisat.org/10737/
 
Title Isolation and characterization of stress inducible promoters from Pennisetum
glaucum and their role in abiotic stress adaptation
 
Creator Divya, K
Reddy, P S
Bhatnagar-Mathur, P
Vadez, V
Sharma, K K
 
Subject Abiotic Stress
 
Description Abiotic stresses including drought, salinity, oxidative stress, extreme
temperatures and a combination of these stresses cause
immense loss to agricultural crop production. These losses could
be immensely reduced if the crops were able to tolerate these
above mentioned conditions. This can be overcome by developing
transgenic plants with stress inducible promoters with
specific genes. Stress-inducible promoters have previously been
identified, but there is a shortage of efficient promoters for gene
expression that display favorable characteristics in their native
plants as well as in xenogeneic species. In the present study five
stress inducible promoters PgApx, PgDhn and PgHsc70, PgLea,
PgHsp10 were isolated and functionally validated in the tobacco
plants. Five stress inducible promoters were isolated using
genome-walking method. Further, all five promoters were subjected
to cis-motif analysis and identified several stress inducible
and tissue specific cis-acting elements. These five full-length
promoters were fused with uidA gene in plant transformation
vector pMDC164 and transferred to tobacco. Putative transgenic
positive plants were confirmed by PCR and positive plants advanced
for T1 and T2 generations. Homozygous T2 transgenic
plants were analysed for their tolerance to these stresses. The
PgApx pro, PgDhn pro, PgHsc70, PgLea, PgHsp10 pro offers
advantages over constitutive promoters to produce genetically
modified tolerant crops to these adverse conditions. In summary,
PgHsc70 pro is active in high temperatures, PgDhn pro in
heat, cold and drought and PgApx pro in drought stress, PgLea
pro in heat and drought, PgHsp10 pro in control, heat, cold, salt
and drought stress treatments.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10737/1/393.pdf
Divya, K and Reddy, P S and Bhatnagar-Mathur, P and Vadez, V and Sharma, K K (2017) Isolation and characterization of stress inducible promoters from Pennisetum glaucum and their role in abiotic stress adaptation. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.