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Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance

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Relation http://oar.icrisat.org/10740/
 
Title Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous
expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought
stress tolerance
 
Creator Somanaboina, A K
Narasu, L N
Varshney, R K
KaviKishor, P B
 
Subject Abiotic Stress
Genetics and Genomics
 
Description Tomato (Solanum lycopersicum L.) is the second most produced
and consumed vegetable, next to potato. Pusa early dwarf (PED)
variety of tomato is sensitive to salinity and drought which reduces
the yield and fruit quality in tomato. In the present study,
tomato transgenics were developed with osmotin-like protein
(OLP) and chitinase (Chi11) genes via in vitro and in planta
transformation methods. Transgene integration and transcript
levels were confirmed by multiplex PCR, DNA blot, and multiplex
reverse transcriptase PCR. Homozygous T2 transgenics,
when evaluated for salt and drought stress, showed enhanced
tolerance compared to wild-type plants. Transgenics showed
enhanced root biomass under normal conditions. Transformants
also displayed higher proline content, K+, relative water content,
chlorophyll fluorescence, total biomass, vascular conductivity,
and fruit yield compared to the wild-type plants under stress
conditions. Co-immunoprecipitation revealed that Chi11 co-expresses
with phosphofructokinase2 (PFK2), which may play a
role in enhanced root biomass. qPCR analysis resulted in higher
transcript levels of OLP, Chi11, and PFK2 in transgenics as compared
to the wild-type plants.
 
Date 2017-02
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10740/1/425.pdf
Somanaboina, A K and Narasu, L N and Varshney, R K and KaviKishor, P B (2017) Development of transgenic tomato (Solanum lycoperscicum L.) by heterologous expression of osmotin-like protein (OLP) and chitinase (Chi11) genes for salt and drought stress tolerance. In: InterDrought-V, February 21-25, 2017, Hyderabad, India.