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Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature

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Relation http://oar.icrisat.org/10758/
http://dx.doi.org/10.1016/j.genrep.2018.05.002
10.1016/j.genrep.2018.05.002
 
Title Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature
 
Creator Mangrauthia, S K
Sailaja, B
Pusuluri, M
Jena, B
Prasanth, V V
Agarwal, S
Senguttuvel, P
Sarla, N
Ravindra Babu, V
Subrahmanyam, D
Voleti, S R
 
Subject Abiotic Stress
Rice
Genetics and Genomics
Plant Physiology
 
Description MicroRNAs are small noncoding regulatory RNAs which control gene expression by mRNA degradation or translational repression. They are significant molecular players regulating important biological processes such as developmental timing and stress response. We report here the discovery of miRNAs derived from ribosomal DNA using the small RNA datasets of 16 deep sequencing libraries of rice. Twelve putative miRNAs were identified based on highly stringent criteria of novel miRNA prediction. Surprisingly, 10 putative miRNAs (mi_7403, mi_8435, mi_12675, mi_4266, mi_4758, mi_4218, mi_8200, mi_4644, mi_14291, mi_16235) originated from rDNA of rice chromosome 9. Expression analysis of putative miRNAs and their target genes in heat tolerant and susceptible rice cultivars in control and high temperature treated seedlings revealed differential regulation of rDNA derived miRNAs. This is the first report of rDNA derived miRNAs in rice which indicates their role in gene regulation during high temperature stress in plants. Further studies in this area will open new research challenges and opportunities to broaden our knowledge on gene regulation mechanisms.
 
Publisher Elsevier
 
Date 2018
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10758/1/2018%20Satendra%20Gene%20Reports%20.pdf
Mangrauthia, S K and Sailaja, B and Pusuluri, M and Jena, B and Prasanth, V V and Agarwal, S and Senguttuvel, P and Sarla, N and Ravindra Babu, V and Subrahmanyam, D and Voleti, S R (2018) Deep sequencing of small RNAs reveals ribosomal origin of microRNAs in Oryza sativa and their regulatory role in high temperature. Gene Reports (TSI), 11. pp. 270-278. ISSN 24520144