<strong>Dual substrate fermentation using palm oil and glucose for production of eco-friendly biosurfactants using P. aeruginosa NITT 6L</strong>
Online Publishing @ NISCAIR
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dc |
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Title Statement |
<strong>Dual substrate fermentation using palm oil and glucose for production of eco-friendly biosurfactants using P. aeruginosa NITT 6L</strong> |
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Added Entry - Uncontrolled Name |
Ambati, Seshagiri Rao; Department of Chemical Engineering, National Institute of Technology, Warangal - 506 004, Telangana State |
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Uncontrolled Index Term |
Pseudomonas aeruginosa; Biosurfactants; ;Rhamnolipids; Process optimization; Fermentation |
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Summary, etc. |
Studies have been conducted to improve biosurfactant (rhamnolipid) production from <em>Pseudomonas aeruginosa</em> NITT 6L in liquid state fermentation utilizing palm oil and glucose as substrates. The <em>p</em>H and minimal salt media (MSM) compositions such as KH<sub>2</sub>PO<sub>4</sub>, K<sub>2</sub>HPO<sub>4</sub>, NaNO<sub>3</sub>, MgSO<sub>4</sub>, NaCl and FeSO<sub>4</sub> are optimized using design of experiments. One-factor-at-a-time optimization is employed to evaluate the effects of palm oil and its co-substrate glucose on biosurfactant production. The optimal levels of the aforementioned variables are (g/L) glucose 30.0, KH<sub>2</sub>PO<sub>4</sub> 2.0, K<sub>2</sub>HPO<sub>4</sub> 5.0, NaNO<sub>3</sub> 3.5, FeSO<sub>4</sub> 0.003, MgSO<sub>4</sub> 0.2, with palm oil concentration of 1.5% (v/v). The fermentation conditions viz. period of fermentation, agitation rate, aeration and concentration of inoculum are optimized by carrying out separate experimentations. The optimum period of fermentation, agitation rate, aeration and concentration of inoculum in the fermentation medium are found to be 7 days, 150 rpm, 60% and 2% (v/v), respectively, for maximum rhamnolipid production of about 3.73 g/RE l. |
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Publication, Distribution, Etc. |
Indian Journal of Chemical Technology (IJCT) 2018-07-05 13:43:53 |
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Electronic Location and Access |
application/pdf http://op.niscair.res.in/index.php/IJCT/article/view/14004 |
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Data Source Entry |
Indian Journal of Chemical Technology (IJCT); ##issue.vol## 25, ##issue.no## 1 (2018): Indian Journal of Chemical Technology |
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Language Note |
en |
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