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Viral metagenomics reveals sweet potato virus diversity in the Eastern and Western Cape provinces of South Africa

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Relation http://oar.icrisat.org/10812/
http://dx.doi.org/10.1016/j.sajb.2018.05.024
10.1016/j.sajb.2018.05.024
 
Title Viral metagenomics reveals sweet potato virus diversity in the Eastern and Western Cape provinces of South Africa
 
Creator Nhlapo, T F
Rees, D J G
Odeny, D A
Mulabisana, J M
Rey, M E C
 
Subject Genetics and Genomics
 
Description Limited studies have been undertaken with regard to virus complexes contributing to the aetiology of sweet potato virus disease (SPVD) in South Africa (SA). In this study, a metagenomic approach was adopted to reveal the genetic diversity of viruses infecting sweet potato. In order to undertake a comprehensive analysis of viral sequences, total RNA was isolated from 17 asymptomatic and symptomatic sweet potato plants that were collected from the Eastern (EC) and Western Cape (WC) provinces of SA. DNase-treated total RNA was depleted of ribosomal RNA (rRNA) and deep-sequenced using the Illumina MiSeq platform. Genomic DNA, isolated from the same plants, underwent rolling circle amplification (RCA) and deep sequencing. Sequence reads were analysed with the CLC Bio Genomics Workbench. Both de novo and reference-guided assemblies were performed resulting in four near full-length RNA virus genomes. BLAST searches using de novo assembled sequences against published virus genomes confirmed the presence of previously detected begomoviruses in the Western Cape (WC) province, namely Sweet potato mosaic virus (SPMaV) and Sweet potato leaf curl Sao Paulo virus (SPLCSPV). The begomoviruses were detected in mixed infections with two major disease-causing RNA viruses, Sweet potato feathery mottle virus (SPFMV) and Sweet potato chlorotic stunt virus (SPCSV). The sequence data further demonstrated mixed infections of RNA and DNA viruses from 11 of the 17 sequenced samples. Metagenomics is a reliable diagnostic tool for virus diversity detection, in particular virus-complexes and synergies affecting disease aetiology.
 
Publisher Elsevier
 
Date 2018-07
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/10812/1/Viral%20metagenomics%20reveals%20sweet%20potato%20virus%20diversity%20in%20the%20Eastern.pdf
Nhlapo, T F and Rees, D J G and Odeny, D A and Mulabisana, J M and Rey, M E C (2018) Viral metagenomics reveals sweet potato virus diversity in the Eastern and Western Cape provinces of South Africa. South African Journal of Botany (TSI), 117. pp. 256-267. ISSN 02546299