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Plasmid Encoded AcrAB–TolC Tripartite Multidrug-Efflux System in Acidiphilium symbioticum H8

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Title Plasmid Encoded AcrAB–TolC Tripartite Multidrug-Efflux
System in Acidiphilium symbioticum H8
 
Creator Singh, Samarendra K.
Singh, Arpita
Banerjee, Pataki C
 
Subject Molecular & Human Genetics
 
Description Acidophilic bacterium, Acidiphilium symbioticum
H8, is resistant to high levels of several heavy metals,
hydrophobic agents, and organic solvents. The *9.6 kb
plasmid pASH8, was purified, digested with HindIII, and
sub-cloned in pUC19 at the respective site. Three different
fragment size clones were achieved. The clones were
completely sequenced and analyzed. The first clone
encodes for a single putative open reading frame (ORF),
which showed significant homology to several rusticyaninA1
proteins. The second clone encodes for a 43-kDa
protein, which has conserved domain homology with several
outer envelop TolC proteins. The clone with pASH8
tolC gene can functionally complement an Escherichia coli
tolC mutant strain, making it resistant to several toxic
hydrophobic agents, earlier for which it was sensitive. The
tolC gene was found to be essential for imparting resistance
to the clone toward these toxic hydrophobic agents. The
third clone encodes for a putative 318-aa AcrA (acriflavine
resistance protein A) protein and the clone was resistance
to plasmid curing dye acriflavine. The clone also has a
truncated ORF, which showed significant homology to
cation-efflux pump AcrB. This study is the first to report a multi-drug efflux system to be encoded on a plasmid of any
Acidiphilium strain.
 
Publisher Springer Verlag
 
Date 2010
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/67/1/CURRENT_MICROBIOLOGY__61(3)163%2D168;2010[37].pdf
Singh, Samarendra K. and Singh, Arpita and Banerjee, Pataki C (2010) Plasmid Encoded AcrAB–TolC Tripartite Multidrug-Efflux System in Acidiphilium symbioticum H8. Current Microbiology, 61. pp. 163-168.
 
Relation htpp://dx.doi.org/10.1007/s00284-010-9590-9
http://www.eprints.iicb.res.in/67/