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Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution

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Title Effects of Arginine and Other Solution Additives on the
Self-Association of Different Surfactants: An Investigation
at Single-Molecule Resolution
 
Creator Haldar, Shubhasis
Chattopadhyay, Krishnananda
 
Subject Structural Biology & Bioinformatics
 
Description Fluorescence correlation spectroscopy is used to
monitor the self-association of SDS and DTAB monomers at
single-molecule resolution. Tetramethylrhodamine-5-maleimide (TMR)
has been chosen as a probe because rhodamine dyes have been
shown to bind surfactant micelles. Correlation functions obtained by
FCS experiments have been fit using conventional discrete diffusional
component analysis as well as the more recent maximum
entropy method (MEM). Hydrodynamic radii calculated from the
diffusion time values increase with surfactant concentration as the
monomers self-associate. Effects of several solution additives on the
self-association property of the surfactants have been studied. Urea
and glycerol inhibit self-association, and arginine shows a dual nature.
With SDS, arginine favors self-association, and with DTAB, it inhibits
micelle formation. We propose surfactant self-association to be a
“supersimplified” model of protein aggregation.
 
Publisher American Chemical Society
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/105/1/LANGMUIR%2C27(10)_5842%2D5849%2C2011[48].pdf
Haldar, Shubhasis and Chattopadhyay, Krishnananda (2011) Effects of Arginine and Other Solution Additives on the Self-Association of Different Surfactants: An Investigation at Single-Molecule Resolution. Langmuir, 27 (10). pp. 5842-5849.
 
Relation http://dx.doi.org/10.1021/la200580z
http://www.eprints.iicb.res.in/105/