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Manganese and Iron both Influence the Shoot Transcriptome of Typha Angustifolia Despite Distinct Preference towards Manganese Accumulation

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Title Manganese and Iron both Influence the Shoot Transcriptome
of Typha Angustifolia Despite Distinct Preference
towards Manganese Accumulation
 
Creator Chakraborty, D
Kumar, S Abhay
Sen, M
Apte, S K
Das, S
Acharya, R
Das, T
Reddy, A V R
Roychoudhury, Susanta
Rajaram, H
Seal, A
 
Subject Molecular & Human Genetics
 
Description Typha angustifolia is a metal hypertolerant
grass that predominates the wetlands of uranium tailings
in Jaduguda, India, contaminated with extreme levels of
iron (Fe) and manganese (Mn). In the paper investigations
were carried out to understand the molecular
mechanism of metal tolerance in this tolerant macrophyte.
Metal analysis was coupled with fluorescent
differential display (FDD) and reverse northern to
compare the metal profile and transcriptome between
Typha plants growing in the tailings versus a control
wetland. The expressions of the validated transcripts in
Mn and Fe stress were confirmed using reverse northern
and semiquantitaive RT-PCR. Typha selectively accumulated
and stored Mn in shoots but excluded/restricted
Fe both in the field and in vitro. Differential expressions
of 23 ESTs were validated among 118 FDD genes.
Although Typha behaved as a Mn accumulator, these
transcripts were found to be regulated in a complex
fashion not only by Mn accumulated in the shoots but
also by Fe excluded from the shoots. Our results
provide the first report in the molecular understanding
of metal tolerance in Typha angustifolia and indicate a
 
Date 2011
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/127/1/PLANT_AND_SOIL%2C342(1%2D2)_301%2D317%2C2011[61].pdf
Chakraborty, D and Kumar, S Abhay and Sen, M and Apte, S K and Das, S and Acharya, R and Das, T and Reddy, A V R and Roychoudhury, Susanta and Rajaram, H and Seal, A (2011) Manganese and Iron both Influence the Shoot Transcriptome of Typha Angustifolia Despite Distinct Preference towards Manganese Accumulation. Plant Soil, 342. pp. 301-317.
 
Relation http://dx.doi.org/10.1007/s11104-010-0696-4
http://www.eprints.iicb.res.in/127/