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Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�

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Title Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�
 
Creator Bhowmick, Rudra
Ghosal, Abhisek
Das, Bhabatosh
Koley, Hemanta
Saha, Dhira Rani
Ganguly, Sandipan
Nandy, Ranjan K
Bhadra, Rupak K
Chatterjee, Nabendu Sekhar
 
Subject Infectious Diseases and Immunology
 
Description The chitin-binding protein GbpA of Vibrio cholerae has been recently described as a common adherence
factor for chitin and intestinal surface. Using an isogenic in-frame gbpA deletion mutant, we first show that V.
cholerae O1 El Tor interacts with mouse intestinal mucus quickly, using GbpA in a specific manner. The gbpA
mutant strain showed a significant decrease in intestinal adherence, leading to less colonization and fluid
accumulation in a mouse in vivo model. Purified recombinant GbpA (rGbpA) specifically bound to N-acetyl-
D-glucosamine residues of intestinal mucin in a dose-dependent, saturable manner with a dissociation constant
of 11.2 �M. Histopathology results from infected mouse intestine indicated that GbpA binding resulted in a
time-dependent increase in mucus secretion. We found that rGbpA increased the production of intestinal
secretory mucins (MUC2, MUC3, and MUC5AC) in HT-29 cells through upregulation of corresponding genes.
The upregulation of MUC2 and MUC5AC genes was dependent on NF-�B nuclear translocation. Interestingly,
mucin could also increase GbpA expression in V. cholerae in a dose-dependent manner. Thus, we propose that
there is a coordinated interaction between GbpA and mucin to upregulate each other in a cooperative manner,
leading to increased levels of expression of both of these interactive factors and ultimately allowing successful
intestinal colonization and pathogenesis by V. cholerae.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/175/1/INFECTION_AND_IMMUNITY%2C76(_11)%2C4968%2D4977%2C2008[19].pdf
Bhowmick, Rudra and Ghosal, Abhisek and Das, Bhabatosh and Koley, Hemanta and Saha, Dhira Rani and Ganguly, Sandipan and Nandy, Ranjan K and Bhadra, Rupak K and Chatterjee, Nabendu Sekhar (2008) Intestinal Adherence of Vibrio cholerae Involves a Coordinated Interaction between Colonization Factor GbpA and Mucin�. Infection and Immunity, 76 (11). pp. 4968-4977.
 
Relation http://dx.doi.org/10.1128/IAI.01615-07
http://www.eprints.iicb.res.in/175/