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In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis

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Title In vitro and in vivo activity of Aloe vera leaf exudate
in experimental visceral leishmaniasis
 
Creator Dutta, Avijit
Sarkar, Debjani
Gurib-Fakim, Ameenah
Mandal, Chitra
Chatterjee, Mitali
 
Subject Infectious Diseases and Immunology
 
Description The leishmanicidal activity of Aloe vera leaf
exudate (AVL) has been demonstrated in promastigotes and
axenic amastigotes, but its effectiveness in animal models
has not been evaluated. The presence of alkaloids,
triterpenes, cyanidines, proanthocyanidines, tannins, and
saponins in AVL was identified. Its effectiveness in four
Leishmania donovani strains was studied both in promastigotes
(IC50 ranged from 70–115 μg/ml) and amastigotes
(IC50 ranged from 3.1–11.4 μg/ml). In amastigotes, the killing by AVL was facilitated through its induction of
nitric oxide in leishmania-infected macrophages. The safety
index was good as AVL up to 300 μg/ml remained nontoxic
to monocytes and macrophages. In a L. donovani
BALB/c mouse model, oral or subcutaneous administration
of AVL (15 mg/kg body weight×5 days) reduced parasitemia
by >90% in the liver, spleen, and bone marrow
without impairment of hepatic and renal functions. Collectively,
we conclude that AVL shows promising antileishmanial
activity and may provide a new lead agent in the
treatment of Leishmaniasis.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/188/1/PARASITOLOGY_RESEARCH%2C102(_6)%2C1235%2D1242%2C2008[94].pdf
Dutta, Avijit and Sarkar, Debjani and Gurib-Fakim, Ameenah and Mandal, Chitra and Chatterjee, Mitali (2008) In vitro and in vivo activity of Aloe vera leaf exudate in experimental visceral leishmaniasis. Parasitol Res, 102 (6). pp. 1235-1242.
 
Relation http://dx.doi.org/10.1007/s00436-008-0899-2
http://www.eprints.iicb.res.in/188/