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Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio

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Title Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio
 
Creator Paul, Sudipta
Mukherjee, Dola
Pramanick, Kousik
Kundu, Sourav
Bhattacharyya, S P
De, Priyanka
Mukherjee, Dilip
 
Subject Molecular & Human Genetics
 
Description The effects of salmon calcitonin (sCT) on the secretion of
17b-estradiol (E2) were examined in female common carp,
Cyprinus carpio. Vitellogenic stage fish adapted to high-Ca
water were i.p. injected with vehicle, sCT, human chorionic
gonadotropin (hCG), or hCG plus sCT. To determine
whether ovarian follicles are equipped with CT receptors, a
CT binding assay was conducted. In the in vitro experiments,
vitellogenic follicles were incubated with stimulators and
inhibitors. Administration of sCT increased the basal and
hCG-stimulated E2 release in vivo and in vitro. Binding
characteristics of [125I]sCT to plasma membrane preparation
of carp ovarian follicles showed saturability with high-affinity
(KdZ48.48 pmol/l and BmaxZ1.2 pmol/mg protein). To
clarify the mechanism of E2 production by sCT, in vitro effect
of sCT and hCG on aromatase activity (conversion of
testosterone to E2) and cytochrome P450 aromatase (P450arom) gene expression in carp ovarian follicles were
investigated. Salmon CT-stimulated both aromatase activity
and P450arom gene expression in ovarian follicles of carp.
sCT-stimulated E2 release by the ovarian follicles in vitro was
augmented in the presence of dibutyryl cAMP. Inhibitor of
protein kinase A (PKA), SQ 22536 inhibited sCT-stimulated
steroid production in a dose-dependent manner. Specific
inhibitor of protein kinase C (PKC), NPC-15437 dihydrochloride
had no inhibitory effects on sCT-induced E2
release. The present study indicates that sCT binds specifically
to carp ovary and stimulates E2 production by increasing the
activity of cytochrome P450 aromatase and P450arom gene
expression. The results further suggest that stimulatory action
of sCTon E2 production is mediated through cAMP pathway.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/233/1/JOURNAL_OF_ENDOCRINOLOGY%2C196(2)%2C413%2D424%2C2008[130].pdf
Paul, Sudipta and Mukherjee, Dola and Pramanick, Kousik and Kundu, Sourav and Bhattacharyya, S P and De, Priyanka and Mukherjee, Dilip (2008) Stimulation of Salmon Calcitonin on Secretion of 17b-Estradiol by the Ovarian Follicles of Common Carp, Cyprinus Carpio. Journal of Endocrinology, 196 (2). pp. 413-424.
 
Relation http://dx.doi.org/10.1677/JOE-07-0188
http://www.eprints.iicb.res.in/233/