Record Details

Quercetin Interferes With iron Metabolism in Leishmania donovani and Targets Ribonucleotide Reductase to Exert Leishmanicidal Activity

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Quercetin Interferes With iron Metabolism in Leishmania donovani and Targets Ribonucleotide Reductase to Exert Leishmanicidal Activity
 
Creator Sen, Gargi
Mukhopadhyay, Sibabrata
Ray, Manju
Biswas, Tuli
 
Subject Cell Biology & Physiology
 
Description The possibility of developing antileishmanial drugs was evaluated by intervention in the
parasite’s iron metabolism, utilizing quercetin (Qr) under in vivo conditions, and identifying the target
of this lipophilic metal chelator against Leishmania donovani.
Methods: Interaction between Qr and serum albumin (SA) was studied by using the intrinsic fluorescence
of Qr as a probe. The effect of treatment with Qr and SA on the proliferation of amastigotes
was determined by evaluating splenic parasite load. Disintegration of parasites in response to combination
treatment was assessed from ultrastructural analysis using a transmission electron microscope.
Quenching of the tyrosyl radical of ribonucleotide reductase (RR) in treated amastigotes was detected
by an electron paramagnetic resonance study.
Results: Treatment with a combination of Qr and SA increased bioavailability of the flavonoid and
proved to be of major advantage in promoting the effectiveness of Qr towards the repression of
splenic parasite load from 75%, P < 0.01 to 95%, P < 0.002. Qr-mediated down-regulation of RR (P
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/241/1/JOURNAL_OF_ANTIMICROBIAL_CHEMOTHERAPY%2C__61(5)%2C_1066%2D1075%2C_2008[85].pdf
Sen, Gargi and Mukhopadhyay, Sibabrata and Ray, Manju and Biswas, Tuli (2008) Quercetin Interferes With iron Metabolism in Leishmania donovani and Targets Ribonucleotide Reductase to Exert Leishmanicidal Activity. Journal of Antimicrobial Chemotherapy, 61 (5). pp. 1066-1075.
 
Relation http://dx.doi.org/10.1093/jac/dkn053
http://www.eprints.iicb.res.in/241/