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DNA Damage Induced p53 Downregulates Cdc20 by Direct Binding to its Promoter Causing Chromatin Remodeling

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Title DNA Damage Induced p53 Downregulates Cdc20
by Direct Binding to its Promoter Causing
Chromatin Remodeling
 
Creator Taraswi , Taraswi
Nath, Somsubhra
Roychoudhury, Susanta
 
Subject Molecular & Human Genetics
 
Description CDC20 is a critical molecule in the Spindle Assembly
Checkpoint (SAC). It activates the Anaphase promoting
complex and helps a dividing cell to proceed
towards Anaphase. CDC20 is overexpressed in
many tumor cells which cause chromosomal
instability. There have been limited reports on the
mechanism of SAC’s response to genotoxic stress.
We show that ectopically expressed p53 or DNA
damage induced endogenous p53 can downregulate
Cdc20 transcriptionally. We have identified a consensus
p53-binding site on the Cdc20 promoter
and have shown that it is being used by p53
to bind the promoter and bring about chromatin
remodeling thereby repressing Cdc20. Additionally,
p53 also downregulates Cdc20 promoter through
CDE/CHR element, but in a p21 independent
manner. This CDE/CHR element-mediated downregulation
occurs only under p53 overexpressed
condition but not in the context of DNA damage.
The present results suggest that the two CCAAT
elements in the Cdc20 promoter are not used by
p53 to downregulate its activity, as reported earlier
 
Publisher Oxford University Press
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/249/1/NUCLEIC_ACIDS_RESEARCH%2C__37_(8)%2C_2688%2D2698%2C2009[73].pdf
Taraswi , Taraswi and Nath, Somsubhra and Roychoudhury, Susanta (2009) DNA Damage Induced p53 Downregulates Cdc20 by Direct Binding to its Promoter Causing Chromatin Remodeling. Nucleic Acids Research, 37 (8). pp. 2688-2698.
 
Relation http://dx.doi.org/10.1093/nar/gkp110
http://www.eprints.iicb.res.in/249/