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9-O-acetylated sialic acids enhance entry of virulent Leishmania donovani promastigotes into macrophages

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Title 9-O-acetylated sialic acids enhance entry of virulent
Leishmania donovani promastigotes into macrophages
 
Creator Ghoshal, Angana
Mukhopadhyay, S
Chava, A. K
Gerwig, G. J
Kamerling, Johannis P
Chatterjee, M
Mandal, Chitra
 
Subject Infectious Diseases and Immunology
 
Description Distribution of 9-O-acetylated sialic acids (9-O-AcSA) on Leishmania donovani has been previously reported. Considering
their role in recognition, the differential distribution of sialic acids especially 9-O-acetylated sialic acids in avirulent (UR6)
versus virulent (AG83 and GE1) promastigotes of Leishmania donovani and its role in entry into macrophages was explored.
Fluorimetric-HPLC, fluorimetric determination and ELISA revealed 14-, 8- and 5-fold lower sialic acids in UR6 as
compared to AG83. Interestingly, on UR6, flow cytometry indicated lower (a2p6)-linked sialoglycoproteins along with
minimal 9-O-acetylated sialoglycoproteins by Scatchard analysis. Further, UR6 demonstrated a 9- and 14.5-fold lower
infectivity and phagocytic index than AG83. Additionally, de-O-acetylation and de-sialylation of AG83 demonstrated a
3- and 1.5-fold reduced phagocytic index. The role of 9-O-AcSA in entry was further confirmed by pre-blocking the
macrophage surface with a cocktail of sugars followed by microscopic quantification. The phagocytic index of AG83
exclusively through 9-O-AcSA was significantly high. Interestingly, AG83 produced higher metacyclic promastigotes
containing increased 9-O-AcSA as compared to avirulent UR6 supporting its virulent nature. Taken together; our results
conclusively demonstrate the increased presence of 9-O-acetylated sialic acid on promastigotes of virulent Leishmania
donovani as compared to avirulent UR6 and their subsequent role in entry within macrophages.
 
Publisher Cambridge University Press
 
Date 2009
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/253/1/PARASITOLOGY%2C136(2)%2C159%2D173%2C2009[113].pdf
Ghoshal, Angana and Mukhopadhyay, S and Chava, A. K and Gerwig, G. J and Kamerling, Johannis P and Chatterjee, M and Mandal, Chitra (2009) 9-O-acetylated sialic acids enhance entry of virulent Leishmania donovani promastigotes into macrophages. Parasitology, 136 (2). pp. 159-173.
 
Relation http://dx.doi.org/10.1017/S0031182008005180
http://www.eprints.iicb.res.in/253/