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gp63 in Stable Cationic Liposomes Confers Sustained Vaccine Immunity to Susceptible BALB/c Mice Infected with Leishmania donovani

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Title gp63 in Stable Cationic Liposomes Confers Sustained Vaccine Immunity to Susceptible BALB/c Mice Infected with Leishmania donovani
 
Creator Bhowmick, Swati
Ravindran, Rajesh
Ali, Nahid
 
Subject Infectious Diseases and Immunology
 
Description Visceral leishmaniasis is deadly if not treated, and development of a vaccine with long-term immunity
remains a challenge. In this study, we showed that cationic distearoyl phosphatidylcholine (DSPC) liposomes,
when used as vaccine adjuvant with the immunodominant 63-kDa glycoprotein (gp63) of Leishmania donovani
promastigotes, induced significant protection against progressive visceral leishmaniasis in susceptible BALB/c
mice. gp63 used without adjuvant elicited partial protection but in association with liposomes exhibited
marked resistance in both the livers and spleens of the mice challenged 10 days after the last vaccination. The
protective efficacy of liposomal gp63 vaccination was dose dependent, with 2.5 �g of protein showing optimal
protection. The immunity conferred by this vaccine formulation was durable, as mice challenged 12 weeks after
immunization were still protected, and the infection was controlled for at least 3 months postchallenge.
Production of gamma interferon (IFN-�) and interleukin-4 (IL-4) by splenic T cells, and of serum immunoglobulin
G1 (IgG1) and IgG2a following immunization, suggested that a mixed Th1/Th2 response had been
induced following immunization. However, control of disease progression and parasitic burden in mice
vaccinated with gp63 in cationic DSPC liposomes was associated with enhancement of antigen-specific IFN-�
and downregulation of IL-4, demonstrating a Th1 bias. Long-term immunity elicited by this vaccine corresponded
to, in addition to the presence of antigen-specific Th1, CD8� T-cell responses. Our results demonstrated
that stable cationic liposomes containing gp63 acted as a potent adjuvant for protein antigen to induce
long-term protection against L. donovani that represents an alternative to DNA vaccination.
 
Date 2008
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/291/1/INFECTION_AND_IMMUNITY%2C76(_3)%2C_1003%2D1015%2C2008[114].pdf
Bhowmick, Swati and Ravindran, Rajesh and Ali, Nahid (2008) gp63 in Stable Cationic Liposomes Confers Sustained Vaccine Immunity to Susceptible BALB/c Mice Infected with Leishmania donovani. Infection and Immunity, 76 (3). pp. 1003-1015.
 
Relation http://dx.doi.org/10.1128/IAI.00611-07
http://www.eprints.iicb.res.in/291/