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Matrix Metalloproteinase-9 Activity and Expression is Reduced by Melatonin During Prevention of Ethanol-induced Gastric Ulcer inB Mice

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Title Matrix Metalloproteinase-9 Activity and Expression is Reduced by Melatonin During Prevention of Ethanol-induced Gastric Ulcer inB Mice
 
Creator Swarnakar, Snehasikta
Mishra, Amartya
Ganguly, Krishnendu
Sharma, Anamika Vivek
 
Subject Cell Biology & Physiology
 
Description Matrix metalloproteinases (MMPs) play an important role in
degradation of gastric extracellular matrix proteins. However, no reports are available on the relationship between the activity of MMPs and gastric ulceration induced by alcohol. Our objective was to investigate the effect of
melatonin (N-acetyl-5-methoxytryptamine) on the regulation of MMP-9 and MMP-2 activities during prevention of ethanol-induced gastric ulcer. Biochemical and zymographic methods were used to analyze MMP-9 and -2 activities in gastric tissues of Balb/c mice following induction of gastric ulcer
by ethanol. Our studies reveal that melatonin arrested cell injury, protein carbonyl formation, and lipid peroxidation in mice during gastroprotection. Melatonin dose-dependently reduced proMMP-9 activity that was induced (� 25-fold) during ethanol-induced gastric ulceration. Severity of gastric ulcers were correlated proportionately with increased dose of ethanol and elevated activity of proMMP-9 and -2. The reduced activities of MMP-9 and -2 were associated with reduced expression of TNF-a and increased
expression of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2). We conclude that melatonin’s ability to prevent ethanol-induced gastric ulceration in mice is related to a reduction in proMMP-9 activity and expression.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/309/1/JOURNAL_OF_PINEAL_RESEARCH%2C43_(1)%2C__56%2D64[56].pdf
Swarnakar, Snehasikta and Mishra, Amartya and Ganguly, Krishnendu and Sharma, Anamika Vivek (2007) Matrix Metalloproteinase-9 Activity and Expression is Reduced by Melatonin During Prevention of Ethanol-induced Gastric Ulcer inB Mice. Journal Pineal Research, 43 (1). pp. 56-64.
 
Relation http://dx.doi.org/10.1111/j.1600-079X.2007.00443.x
http://www.eprints.iicb.res.in/309/