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Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions

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Title Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor
strains isolated before, during and after the O139 outbreak
based on the intergenomic heterogeneity of the 16S-23S rRNA
intergenic spacer regions
 
Creator Ghatak, Atreyi
Majumder, Anasuya
Ghosh, Ranjit K
 
Subject Molecular & Human Genetics
 
Description We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region
(ISR) associated with the eight rrn operons (rrna-rrnh) of Vibrio cholerae serogroup O1 El Tor strains isolated
before, during and after the O139 outbreak. ISR classes ‘a’ and ‘g’ were found to be invariant, ISR-B (ISRb and
ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the maximum
variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the
pre-O139 serogroup and post-O139 serogroup O1 El Tor strains arose out of two independent clones, which was
congruent with the observation made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of
all five ISR classes, could be successfully used to study phylogeny in this organism.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/322/1/JOURNAL_OF_BIOSCIENCES_30(_5_)_619%2D6252005[6].pdf
Ghatak, Atreyi and Majumder, Anasuya and Ghosh, Ranjit K (2005) Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions. Journal of Bioscience, 30 (5). pp. 619-625.
 
Relation http://dx.doi.org/10.1007/BF02703562
http://www.eprints.iicb.res.in/322/