Record Details

Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor
strains isolated before, during and after the O139 outbreak
based on the intergenomic heterogeneity of the 16S-23S rRNA
intergenic spacer regions
 
Creator Ghatak, Atreyi
Majumder, Anasuya
Ghosh, Ranjit K
 
Subject Molecular & Human Genetics
 
Description We have cloned, sequenced and analysed all the five classes of the intergenic (16S-23S rRNA) spacer region
(ISR) associated with the eight rrn operons (rrna-rrnh) of Vibrio cholerae serogroup O1 El Tor strains isolated
before, during and after the O139 outbreak. ISR classes ā€˜aā€™ and ā€˜gā€™ were found to be invariant, ISR-B (ISRb and
ISRe) exhibited very little variation, whereas ISR-C (ISRc, ISRd, and ISRf) and ISRh showed the maximum
variation. Phylogenetic analysis conducted with all three ISR classes (ISR-B, ISR-C and ISRh) showed that the
pre-O139 serogroup and post-O139 serogroup O1 El Tor strains arose out of two independent clones, which was
congruent with the observation made by earlier workers suggesting that analyses of ISR-C and ISR-h, instead of
all five ISR classes, could be successfully used to study phylogeny in this organism.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/322/1/JOURNAL_OF_BIOSCIENCES_30(_5_)_619%2D6252005[6].pdf
Ghatak, Atreyi and Majumder, Anasuya and Ghosh, Ranjit K (2005) Molecular phylogenetic analysis of Vibrio cholerae O1 El Tor strains isolated before, during and after the O139 outbreak based on the intergenomic heterogeneity of the 16S-23S rRNA intergenic spacer regions. Journal of Bioscience, 30 (5). pp. 619-625.
 
Relation http://dx.doi.org/10.1007/BF02703562
http://www.eprints.iicb.res.in/322/