ICAR Research Data Repository for Knowledge Management
Regulation and Structural Organization of Multimeric Proteins
EPrints@IICB
View Archive Info| Field | Value | |
| Title |
Regulation and Structural Organization of Multimeric Proteins
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| Creator |
Nupur, Banerjee
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| Subject |
Structural Biology & Bioinformatics
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| Description |
purified proteins, by which, the mechanism of a protein’s activity and regulation can be studied under controlled environments. Protein purification, therefore, is the primary prerequisite for in vitro studies. Different approaches are being adopted to purify a protein up to homogeneity, starting from cell lysis, by which the whole cellular content is released into a solution forming ‘cell lysate’. This is then purified by different degrees of ultracentrifugation, precipitation of proteins by ‘salting out’, various types of chromatographic, electrophoretic and spectroscopic techniques involving different properties of the protein, etc. Nowadays, the most popular and simplest way to obtain large amount of sufficiently pure protein is genetic engineering, e. g., the ‘his-tag’ method. |
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| Date |
2010
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| Type |
Thesis
NonPeerReviewed |
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| Format |
application/pdf
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| Identifier |
http://www.eprints.iicb.res.in/339/1/Thesis%2DFinal.pdf
Nupur, Banerjee (2010) Regulation and Structural Organization of Multimeric Proteins. PhD thesis, Jadavpur University. |
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| Relation |
http://www.eprints.iicb.res.in/339/
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