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A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes

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Title A Lupinoside prevented fatty acid induced inhibition of insulin
sensitivity in 3T3 L1 adipocytes
 
Creator Dey, Debleena
Pal, Bikas C
Biswas, Tanushree
Roy, Sib Sankar
Bandyopadhyay, Arun
Mandal, Swapan Kumar
Giri, Bir Bhanu
Bhattacharya, Samir
 
Subject Cell Biology & Physiology
 
Description The decrease in insulin sensitivity to target
tissues or insulin resistance leads to type 2 diabetes
mellitus, an insidious disease threatening global health.
Numerous evidences made free fatty acids (FFAs)
responsible for insulin resistance and type 2 diabetes.
We demonstrate here that the damage of insulin acitivity
by a free fatty acid, palmitate could be prevented
by a lupinoside. An incubation of 3T3 L1 adipocytes
with a FFA i.e. palmitate inhibited insulin stimulated
uptake of 3H-2 deoxyglucose (2 DOG) significantly.
Addition of a lupinoside purified from Pueraria tuberosa,
lupinoside PA4 (LPA4) strongly prevented this
inhibition. We then examined insulin signaling pathway
where palmitate significantly inhibited insulin
stimulated phosphorylation of Insulin receptor tyrosine
kinase, IRS 1and PI3 kinase, PDK1 and Akt/PKB.
LPA4 rescued this inhibition of signaling molecule by
palmitate. Insulin mediated translocation of Glut4, the
glucose transporter in insulin target cells, was effectively
blocked by palmitate while, LPA4 waived this
block. Administration of LPA4 to nutritionally induced
diabetic rats significantly reduced the increase in plasma glucose. All these indicate LPA4 to be a
potentially therapeutic agent for insulin resistance and
type 2 diabetes.
 
Date 2007
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/342/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C_300_(_1%2D2)%2C__149%2D157[81].pdf
Dey, Debleena and Pal, Bikas C and Biswas, Tanushree and Roy, Sib Sankar and Bandyopadhyay, Arun and Mandal, Swapan Kumar and Giri, Bir Bhanu and Bhattacharya, Samir (2007) A Lupinoside prevented fatty acid induced inhibition of insulin sensitivity in 3T3 L1 adipocytes. Mol Cell Biochem, 300 (1-2). pp. 149-157.
 
Relation http://dx.doi.org/10.1007/s11010-006-9378-1
http://www.eprints.iicb.res.in/342/