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Differential Expression of Procollagen Lysine 2- Oxoglutarate 5-Deoxygenase and Matrix Metalloproteinase Isoforms in Hypothyroid Rat Ovary and Disintegration of Extracellular Matrix

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Title Differential Expression of Procollagen Lysine 2-
Oxoglutarate 5-Deoxygenase and Matrix
Metalloproteinase Isoforms in Hypothyroid Rat Ovary
and Disintegration of Extracellular Matrix
 
Creator Saha, Samir Kumar
Ghosh, Pamela
Konar, Aditya
Bhattacharya,, Samir
Roy, Sib Sankar
 
Subject Cell Biology & Physiology
 
Description Hypothyroid-induced reproductive malfunction in both the
sexes is a common phenomenon of global concern. In an attempt
to characterize the differentially expressed genes that
might be responsible for these disorders, we have identified a
number of clones in hypothyroid rat ovary by subtractive
hybridization. One such clone is procollagen lysyl hydroxylase2
(Plod-2), the key enzyme for the first step of collagen
biosynthetic pathway, which was down-regulated in hypothyroid
condition. We have also demonstrated the reduced expression
of other isoforms of Plods, namely Plod-1 and -3 in
hypothyroid rat ovary. The current studies are the first of their kind to report that thyroid hormone regulates the Plod
gene in rat ovary. Moreover, we have shown the up-regulation
of matrix-degrading enzyme(s), matrix metalloproteinase(s)
in the hypothyroid rat ovary, whereas the tissue-inhibitory
metalloproteinase is down-regulated. Finally, the results of
the present studies indicate that in hypothyroid condition,
collagen biosynthesis in ovary seems to be disturbed with
concomitant enhancement in collagen degradation, resulting
in disintegration of overall ovarian structure. (Endocrinology
146: 2963–2975, 2005)
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/360/1/ENDOCRINOLOGY__146(7_)2963%2D2975_;2005[48].pdf
Saha, Samir Kumar and Ghosh, Pamela and Konar, Aditya and Bhattacharya,, Samir and Roy, Sib Sankar (2005) Differential Expression of Procollagen Lysine 2- Oxoglutarate 5-Deoxygenase and Matrix Metalloproteinase Isoforms in Hypothyroid Rat Ovary and Disintegration of Extracellular Matrix. Endocrinology, 146 (70). pp. 2963-2975.
 
Relation http://dx.doi.org/10.1210/en.2004-1440
http://www.eprints.iicb.res.in/360/