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Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection

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Title Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection
 
Creator Dey, Runu
Bhattacharya, Jharna
Datta, Salil C
 
Subject Drug Development/Diagnostics & Biotechnology
Infectious Diseases and Immunology
 
Description A calcium-activated protease caldonopain in the cytosolic fraction of Leishmania donovani has been found to digest different endogenous proteins when subjected to SDS-PAGE. Gelatin-embedded gel electrophoresis confirms presence of calciumdependent protease activity. Ca2+ affects proteolytic activity after 10 h. When host–parasite interaction was conducted in vitro, caldonopain was found to be active after 10 h of incubation with calcium. A 67-kDa protein is specifically digested during this
time and two new proteins of 45 and 36 kDa appeared in SDS-PAGE electrophoregram. This belated action of calcium towards protease activity may be pre-requisite to facilitate invasion of host tissues and thereby mediate protein metabolism during survival of this pathogen both independently and intracellularly. It is likely that calcium metabolism in promastigotes and amastigotes
does not propagate in the same manner. Involvement of calcium to initiate caldonopain activity may be critically associated with signal transduction pathways which may be responsible for the pathobiological action of this parasite. We propose that caldonopain could be a potential target to develop new chemotherapeutic approach against leishmaniasis.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/426/1/MOLECULAR_AND_CELLULAR_BIOCHEMISTRY%2C_281(_1%2D2)%2C_27%2D33_[153].pdf
Dey, Runu and Bhattacharya, Jharna and Datta, Salil C (2006) Calcium-dependent proteolytic activity of a cysteine protease caldonopain is detected during Leishmania infection. Molecular and Cellular Biochemistry, 281 (1-2). pp. 27-33.
 
Relation http://dx.doi.org/ 10.1007/s11010-006-0171-y
http://www.eprints.iicb.res.in/426/