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Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal

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Title Production and purification of a bioactive substance
inhibiting multiple drug resistant bacteria and human
leukemia cells from a salt-tolerant marine Actinobacterium
sp. isolated from the Bay of Bengal
 
Creator Sah, Malay
Jaisankar , P
Das, Satadal
Sarkar, Kalyan K
Roy, Soma
Besra, Shila E
Vedasiromani, J R
Ghosh, Debashish
Sana, Barindra
Mukherjee, Joydeep
 
Subject Drug Development/Diagnostics & Biotechnology
 
Description Four marine actinobacteria tolerant to
200 g NaCl l)1 were screened for antibacterial
activity against eight patient-derived multiple
drug resistant (MDR) bacteria. The active compound
(MW 300.2, predicted molecular formula
C20H28O2) from an actinobacterium, was inhibitory
to three Gram-positive and three Gramnegative
MDR bacteria, seven non-clinical
Gram-positive, four Gram-negative bacteria and
five fungi (MIC: 3.5–4.0 lg ml)1). Also, 54% of
human leukemia (HL-60) cells were killed by the
compound at 0.05 lg ml)1. Bioreactor production
demonstrated unusual primary metabolite kinetics.
Molecular phylogenetic analysis showed this
typical intertidal inhabitant to be a member of
the Streptomyces genus and distinct from other
salt-tolerant actinobacteria. As no compound was found to match the properties in several electronic
databases, our screening strategy should
increase the possibility of discovering bioactive
molecules from rare actinobacteria.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/428/1/BIOTECHNOLOGY_LETTERS%2C__28(_14)%2C__1083%2D1088_[67].pdf
Sah, Malay and Jaisankar , P and Das, Satadal and Sarkar, Kalyan K and Roy, Soma and Besra, Shila E and Vedasiromani, J R and Ghosh, Debashish and Sana, Barindra and Mukherjee, Joydeep (2006) Production and purification of a bioactive substance inhibiting multiple drug resistant bacteria and human leukemia cells from a salt-tolerant marine Actinobacterium sp. isolated from the Bay of Bengal. Biotechnol Lett, 28 (14). pp. 1083-1088.
 
Relation htpp://dx.doi.org/10.1007/s10529-006-9054-2
http://www.eprints.iicb.res.in/428/