Record Details

Variable Degree of Alternative Complement Pathway–Mediated Hemolysis in Indian Visceral Leishmaniasis Induced by Differential Expression of 9-O–Acetylated Sialoglycans

EPrints@IICB

View Archive Info
 
 
Field Value
 
Title Variable Degree of Alternative Complement
Pathway–Mediated Hemolysis in Indian Visceral
Leishmaniasis Induced by Differential Expression
of 9-O–Acetylated Sialoglycans
 
Creator Chava, Anil Kumar
Chatterjee, Mitali
Sharma, Vineeta
Sundar, Shyam
Mandal, Chitra
 
Subject Infectious Diseases and Immunology
 
Description Increased expression of linkage-specific 9-O–acetylated sialoglycans (9-O-AcSGs) has been demonstrated
on erythrocytes from patients with visceral leishmaniasis (VL) by use of Achatinin-H. We assessed the
capacity of this glycotope to influence hemolysis via activation of the alternative complement pathway in patients
with VL, compared with that in healthy control subjects.
Methods. The differential expression of 9-O-AcSGs on surfaces of erythrocytes was measured, 9-O-AcSGs
were affinity purified, and the molecular determinants were identified byWestern blotting. The degree of alternative
complement pathway–mediated hemolysis was compared with expression of 9-O-AcSGs on erythrocytes.
Results. Enhanced expression of linkage-specific 9-O-AcSGs was demonstrated on erythrocytes from patients
with active VL. Six distinct molecular determinants present only on diseased erythrocytes were affinity purified
and were absent after elimination of parasite burden. A correlation ( ) was observed between the presence 2 r p0.9
of 9-O-AcSGs and the degree of alternative complement pathway–mediated hemolysis.
Conclusion. The 9-O-AcSGs expressed on erythrocytes from patients with VL are potent complement activators,
causing enhanced hemolysis via activation of the alternative complement pathway, and may account for
the anemia that is a common manifestation of VL.
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/461/1/JOURNAL_OF_INFECTIOUS_DISEASES%2C189(7)%2C_1257%2D1264[69].pdf
Chava, Anil Kumar and Chatterjee, Mitali and Sharma, Vineeta and Sundar, Shyam and Mandal, Chitra (2004) Variable Degree of Alternative Complement Pathway–Mediated Hemolysis in Indian Visceral Leishmaniasis Induced by Differential Expression of 9-O–Acetylated Sialoglycans. The Journal of Infectious Diseases , 189 (7). pp. 1257-1264.
 
Relation http://dx.doi.org/10.1086/382752
http://www.eprints.iicb.res.in/461/