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Deletion mapping of chromosome 13q in head and neck squamous cell carcinoma in Indian patients: correlation with prognosis of the tumour

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Title Deletion mapping of chromosome 13q in head and neck squamous
cell carcinoma in Indian patients: correlation with prognosis of the
tumour
 
Creator Sabbir, Md. Golam
Roy, Anup
Mandal, Syamsundar
Dam, Aniruddha
Roychoudhury, Susanta
Panda, Chinmay Kumar
 
Subject Molecular & Human Genetics
 
Description Deletions in chromosome (chr.) 13q occur frequently in head and neck squamous cell
carcinoma (HNSCC). Previous studies failed to identify common deleted regions in
chr.13q, though several candidate tumour suppressor genes (TSGs) loci, e.g. BRCA2,
RB1 and BRCAX have been localized in this chromosome, as well as no prognostic
significance of the deletion has been reported. Thus, in the present study, deletion
mapping of chr. 13q has been done in 55 primary HNSCC samples of Indian patients
using 11 highly polymorphic microsatellite markers of which three were intragenic to
BRCA2 gene, one intragenic to RB1 gene and another from BRCAX locus. The
deletion in chr.13q was significantly associated with progression of HNSCC. High
frequencies (27–39%) of loss of heterozygosity were found in 13q13.1 (BRCA2),
13q14.2 (RB1), 13q21.2–22.1 (BRCAX) and 13q31.1 regions. Deletions in the
BRCA2 and RB1 regions were significantly correlated. The four highly deleted regions
were associated with clinical stage and histological grades of the tumour as well as
poor patient outcome. Deletion in the 13q31.1 region was only found to be associated
with HPV infection. High frequencies (11–23%) of microsatellite size alteration (MA)
were seen to overlap with the highly deleted regions. Forty per cent of the samples
showed rare biallelic alteration whereas loss of normal copy of chromosome 13q was
seen in five tumours. Thus, it seems that the putative TSGs located in the BRCAX and
13q31.1 regions as well as the BRCA2 and RB1 genes may have some cumulative effect
in progression and poor prognosis of HNSCC. Significant association between deletion
in BRCA2 and RB1 gene loci may indicate functional relationship between the genes in
this tumour progression.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/474/1/INTERNATIONAL_JOURNAL_OF_EXPERIMENTAL_PATHOLOGY%2C_87(_2)%2C_151%2D161%2C_2006_[104].pdf
Sabbir, Md. Golam and Roy, Anup and Mandal, Syamsundar and Dam, Aniruddha and Roychoudhury, Susanta and Panda, Chinmay Kumar (2006) Deletion mapping of chromosome 13q in head and neck squamous cell carcinoma in Indian patients: correlation with prognosis of the tumour. INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY , 87 (2). pp. 151-161.
 
Relation htpp://dx.doi.org/10.1111/j.0959-9673.2006.00467.x
http://www.eprints.iicb.res.in/474/