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Mahanine InhibitsGrowth and InducesApoptosis in ProstateCancerCellsThroughtheDeactivation of Akt andActivation ofCaspases

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Title Mahanine InhibitsGrowth and InducesApoptosis in
ProstateCancerCellsThroughtheDeactivation of
Akt andActivation ofCaspases
 
Creator Sinha, Swati
Pal, Bikas C
Jagadeesh, Shankar
Banerjee, Partha P
Bandyopadhaya, Arun
Bhattacharya, Samir
 
Subject Cell Biology & Physiology
 
Description The present study was undertaken to evaluate anti-proliferative and -
apoptotic activities of mahanine, a plant derived carbazole alkaloid, in prostate cancer cells and
to determine its molecular mechanism by which it induces apoptotic cell death.
The growth inhibitory and apoptotic inductive effect of mahanine on prostate
cancer cells were examined by measuring cell proliferation and BrdU labeling, caspase activity,
DNA fragmentation, and Western blot analyses.
Mahanine inhibited growth of PC3 and LNCaP prostate cancer cells in a dose and
time-dependent manner. Mechanistically, mahanine inhibited cell-survival pathway by
dephosphorylation of PIP3 dependent kinase 1 (PDK1) thereby deactivation of Akt and
downregulation of Bcl-xL. In addition, mahanine activated caspase pathway (caspases 9 and 3)
and eventually cleavage of DNA repair enzyme, PARP resulting DNA fragmentation and
apoptosis.
Mahanine inhibits growth and induces apoptosis in both androgenresponsive,
LNCaP and androgen-independent, PC3 cells by targeting cell survival pathway.
Prostate 66: 1257–1265, 2006. #2006 Wiley-Liss, Inc.
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Identifier http://www.eprints.iicb.res.in/483/1/PROSTATE%2C__66(_12)%2C_1257%2D1265_[41].pdf
Sinha, Swati and Pal, Bikas C and Jagadeesh, Shankar and Banerjee, Partha P and Bandyopadhaya, Arun and Bhattacharya, Samir (2006) Mahanine InhibitsGrowth and InducesApoptosis in ProstateCancerCellsThroughtheDeactivation of Akt andActivation ofCaspases. The Prostate, 66 (12). pp. 1257-1265.
 
Relation http://dx.doi.org/
http://www.eprints.iicb.res.in/483/